Stable transfection of Acanthamoeba castellanii
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چکیده
منابع مشابه
Lipids of Acanthamoeba Castellanii
The lipids of Acanthamoeba castellanii (Neff) consist of 52% neutral lipids and 48% polar lipids. Triglycerides account for 75% and free sterols for 17% of the neutral lipids. The major phospholipids are phosphatidylcholine (45%), phosphatidylethanolamine (33%), phosphatidylserine (10%), a phosphoinositide (6%), and diphosphatidylglycerol (4%). The phosphoinositide is unique in that it contains...
متن کاملAcanthamoeba castellanii STAT Protein
STAT (signal transducers and activators of transcription) proteins are one of the important mediators of phosphotyrosine-regulated signaling in metazoan cells. We described the presence of STAT protein in a unicellular, free-living amoebae with a simple life cycle, Acanthamoeba castellanii. A. castellanii is the only, studied to date, Amoebozoan that does not belong to Mycetozoa but possesses S...
متن کاملPinocytosis in Acanthamoeba Castellanii
The uptake of radioactively labeled albumin, inulin, leucine, and glucose by Acanthamoeba castellanii (Neff strain) was measured. The uptake is linear with time and appears to be continuous under the conditions of these experiments. Uptake is abolished at 0 degrees C. No evidence for saturation of the uptake mechanism was obtained with either albumin or leucine. Each of the four tracer molecule...
متن کاملPhotoinhibition of Growth in Acanthamoeba castellanii Cultures1
Light from 350 to 680 nm at intensities up to 1.62 X 105 ergs per sec per cm2 slowed exponential growth and lowered the maximum yield in axenic cultures of Acanthamoeba castellanii. Photoinhibition was a linear function of light intensity up to 1.25 X 106 ergs per sec per cm2. At higher intensities, growth was too slow to be measured accurately. A photochemical change occurring in the growth me...
متن کاملThe Fine Structure of Acanthamoeba Castellanii
The fine structure of the trophozoite of Acanthamoeba castellanii (Neff strain) has been studied. Locomotor pseudopods, spikelike "acanthopodia," and microprojections from the cell surface are all formed by hyaline cytoplasm, which excludes formed elements of the cell and contains a fine fibrillar material. Golgi complex, smooth and rough forms of endoplasmic reticulum, digestive vacuoles, mito...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
سال: 2005
ISSN: 0167-4889
DOI: 10.1016/j.bbamcr.2004.08.014